Figure 10.

Nuclear matrix distribution of Sp1 and Sp3 relatively to ERα with or without estradiol treatment. MCF-7 cells were grown on coverslips for 4 d with phenol red free DMEM complemented with 7% 2× calf serum-fetal bovine serum. After ethanol (A and B) or 10 nM estradiol (C and D) treatment for 60 min, the nuclear matrices were prepared and double labeled with anti-Sp1 or anti-Sp3 and anti-ERα antibodies. Spatial distribution was visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merged images signifies colocalization. The merged images were analyzed by the generation of a horizontal linescan (top box) and a diagonal linescan (bottom box) along with a correlation scatter plot. Bar, 5 μm.