Figure 11.

Association of Sp1 and Sp3 with the TFF1 promoter. (A) ChIP and re-ChIP experiments were performed on chromatin prepared from ZR-75 cells treated with ethanol (–) or 10 nM estradiol (+) for 30 min. The antibodies were used as indicated. No antibody: primary antibody was omitted. Control immunoprecipitations with normal rabbit IgG nonspecific antibodies did not produce a PCR product (our unpublished data). Input, ChIP, and re-ChIP DNAs were analyzed by PCR using TFF1 promoter specific primers. This is a representative result of an experiment repeated three times. (B) Quantitation of ChIP and re-ChIP assay PCR products corrected with the input data were determined to evaluate the binding of ERα, Sp1, Sp3, and Sp1-ERα to the TFF1 promoter region. Error bars show the SD (n = 3).