Figure 3.

Nuclear distribution of Sp1 and Sp3 in HeLa, T47D and ZR-75 cells. Fixed HeLa, T47D, and ZR-75 cells were double labeled with anti-Sp1 and anti-Sp3 antibodies. Sp1 and Sp3 distributions were visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merged images signifies colocalization. Boxed areas of each image are shown enlarged as green signal only (top insets), red signal only (middle insets), or merged signals (bottom insets). Bar, 5 μm.