Figure 5.

Sp1 and Sp3 distribution relative to HDAC1 and HDAC2. MCF-7 cells were grown on coverslips in estrogen complete medium, fixed, and double labeled with anti-Sp1 or anti-Sp3 and anti-HDAC1 or anti-HDAC2 antibodies. Spatial distribution was visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merged images signifies colocalization. The merged images were analyzed by the generation of a horizontal linescan (top box) and a diagonal linescan (bottom box) along with a correlation scatter plot. Relative distributions of Sp1 and HDAC1 (A), Sp1 and HDAC2 (B), and Sp3 and HDAC2 (C). Bar, 5 μm.