Figure 9.

Nuclear matrix distribution of Sp1 and Sp3. MCF-7 cells were grown on coverslips in estrogen complete medium, extracted as described in Materials and Methods, and the remaining nuclear matrix was double labeled with anti-Sp1 and anti-Sp3 or anti-HDAC1 and anti-HDAC2 antibodies. (A) DNA was stained by DAPI. Sp1 and Sp3 as well as HADC1 and HDAC2 distributions were visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merged images signifies colocalization. (B-C) The Sp1/Sp3 and HDAC1/HDAC2 merged images were analyzed by the generation of a horizontal linescan (top box) and a diagonal linescan (bottom box) along with a correlation scatter plot. Bar, 5 μm.