Figure 1.

(A) Schematic representation of aconitase variants; wild-type Aco1, Aco1-ΔSP lacking the mitochondrial targeting sequence (16 a.a. indicated in black), Aco1-α containing 85 a.a. of the β-galactosidase α fragment (indicated with dots) inserted after the last amino acid of aconitase, and Aco1-Ura3 containing 358 a.a. of a Ura3 derivative (indicated with diagonal lines) inserted after the last amino acid of aconitase. (B) Subcellular fractionation of yeast cells expressing aconitase variants. Equivalent portions from fractions of the total (T) cytosol (C) and mitochondria (M) were analyzed by Western blotting using antibodies against the indicated proteins.