Table 1.
List of plasmids used in this study
| Plasmids | Fusion protein expressed | Description |
|---|---|---|
| Yeast two-hybrid plasmids | ||
| pSW3017 | GBD-hGle1B | Rayala et al. (2004) |
| pSW3018 | GBD-hGle1B478-698 | hGle1B aa 478-698 in fusion with GBD |
| pSW938 | GBD-hGle1A | Rayala et al. (2004) |
| CP3068 | GAD-hCG1 | hCG1 full-length in fusion with GAD |
| CP3069 | GAD-hCG1190-423 | hCG1 aa 190-423 in fusion with GAD |
| pSW3072 | GAD-hCG1366-423 | hCG1 aa 366-423 in fusion with GAD |
| pSW3073 | GAD-hCG11-165 | hCG1 aa 1-165 in fusion with GAD |
| pSW3074 | GAD-hCG1166-204 | hCG1 aa 166-204 in fusion with GAD |
| pSW3075 | GAD-hCG1205-365 | hCG1 aa 205-365 in fusion with GAD |
| CP77 | GBD-Snf1 | Gift from M. Carlson (Yang et al., 1992) |
| Prokaryotic expression plasmids | ||
| pSW3071 | GST-hCG1 | Full-length hCG1 in fusion with GST |
| pSW3077 | GST-hCG11-365 | hCG1 aa 1-365 in fusion with GST |
| pSW3084 | GST-hCG1366-423 | hCG1 aa 366-423 in fusion with GST |
| pSW3138 | His-hCG11-165 | hCG1 aa 1-165 in fusion with 6× His |
| pSW3101 | His-hGle1548-698 | hGle1B aa 548-698 in fusion with 6× His |
| pSW3102 | His-hGle1655-698 | hGle1B aa 655-698 in fusion with 6× His |
| pSW1413 | GST-hGle1483-559 | hGle1 aa 483-559 in fusion with GST |
| pSW3087 | GST-hCG1366-403 | hGle1 aa 366-403 in fusion with GST |
| pSW3086 | GST-hCG1366-383 | hGle1 aa 366-383 in fusion with GST |
| pSW3089 | GST-hCG1379-423 | hGle1 aa 379-423 in fusion with GST |
| pSW3088 | GST-hCG1403-423 | hGle1 aa 403-423 in fusion with GST |
| CP336 | GST-GFP | Gift from I. Macara (Richards et al., 1996) |
| Eukaryotic expression plasmids | ||
| pSW3099 | hNup155 | Full-length hNup155 |
| pSW3079 | hCG1-GFP | Full-length hCG1 in fusion with EGFP |
| pSW3110 | hGle1B | hGle1B full-length in pCDNA3 vector |
| pSW1406 | hGle1A | hGle1A full-length in pCDNA3 vector |
| pSW3080 | hCG11-365-GFP | hCG1 aa 1-365 in fusion with EGFP |
| pSW3081 | hCG1366-423-GFP | hCG1 aa 366-423 in fusion with EGFP |