Figure 4.

Tyrosine phosphorylation of PKL is required for binding to paxillin. (A) GFP-PKL or the GFP-PKL Triple YF mutant were expressed in CHO.KI cells and plated on FN for 60 min and then immunoprecipitated with anti-GFP antibody. The precipitates were subjected to anti-phosphotyrosine 4G10, GFP, and paxillin immunoblotting (IB). Only the tyrosine phosphorylated GFP-PKL coprecipitated with paxillin, consistent with a requirement for tyrosine phosphorylation of PKL in focal adhesion targeting via an interaction with Paxillin. (B) The experiment was repeated in HEK293A cells with similar results.