Figure 9.

Expression of GFP-PKL TripleYF increases cell spreading similar to cells expressing paxillin ΔLD4. (A) Normal MEFs were transiently transfected with GFP, GFP-PKL WT, GFP-PKL TripleYF, GFP-Paxillin WT, or GFP-Paxillin ΔLD4 followed by plating on 5 μg/ml fibronectin and acquisition of time-lapse images for 60 min. Both GFP-PKL TripleYF and GFP-Paxillin ΔLD4 increased cell spreading, whereas GFP-PKL WT inhibited cell spreading. GFP or GFP-Paxillin expression was without significant effect. (B) The average change in cell area at 10-min intervals between 20 and 60 min postspreading was calculated. Bars, 10 μm. ^**p < 0.01, ^*p < 0.05