Skip to main content
. 2005 Sep;16(9):4341–4349. doi: 10.1091/mbc.E05-04-0281

Figure 1.

Figure 1.

Conditional expression of TgAMA1-myc. (A) Immunofluorescence localization of TgAMA1 and TgAMA1-myc in wild-type (AMA1), AMA1/AMA1-myc, and conditional knockout (Δama1/AMA1-myc) parasites after a 24-h incubation with or without Atc. In the AMA1/AMA1-myc parasites, TgAMA1-myc shows Atc-regulated expression and an apical distribution that is indistinguishable from TgAMA1 in the AMA1 parasites. No TgAMA1, myc-tagged or endogenous, is detected in the knockout parasites after Atc treatment. (B) AMA1, AMA1/AMA1-myc, and Δama1/AMA1-myc parasites were each grown with or without Atc for 24 h, extracted, and probed on Western blots with an anti-TgAMA1 antibody. Only the relevant (60-70 kDa) portion of the blot is shown. The combined TgAMA1 and TgAMA1-myc expression levels in each of the parasite lines were determined by quantitative Western blotting; average results from multiple independent experiments (see Materials and Methods), relative to the AMA1 parasites, are shown below each lane.