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. 2005 Sep;16(9):4350–4361. doi: 10.1091/mbc.E05-04-0304

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Copyright © 2005, The American Society for Cell Biology

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Figure 5. — Morphological alterations induced in Xenopus embryos after injection of Fxr1.Morpholino (Fxr1.MO). (a) Lateral and dorsal views of control embryos for the diffusion of the fluorescein tagged Morpholino directed against the human globin (hGlob.MO) injected in one blastomere of two-cell stage embryos. (b) Injected embryos with xFxr1.MO mismatch (mm) as control, and with increasing concentrations of match MO. Number of injected embryos (n =) is on the right of each panel, as well as percentage of penetrance of the depicted phenotypes. The severity of morphological alterations is proportional to the concentration of injected Fxr1.MO (0.3-1.0 pmol). (c) Rescue of the phenotypes is dose-dependent of the injected HA.Fxr1 mRNA.