Table 1.
Myospecific mRNAs affected by xFxr1 mRNA knockdown in Xenopus embryos stage 30
| Clone ID on chip | Fold of change | BLAST best hit | Similarity | GenBank ID |
|---|---|---|---|---|
| Striated muscle-skeletal | ||||
| Xl.16736.1.S1_at | 3.02 | Myosin binding subunit 85 [hs] | + | BU906692 |
| Xl.15784.1.A1_at | 2.57 | Voltage-dependent calcium channel γ-1 subunit [hs] | + | BJ078880 |
| Xl.1285.2.S1_x_at | −2.50 | Myosin light chain [xl] | = | BG346399 |
| Xl.8919.3.S1_x_at | −2.50 | Tubulin α6 [xl] | = | CD360597 |
| Xl.21921.1.S1_at | −2.52 | Myozenin 1 [xl] | = | BC042928.1 |
| Xl.1285.2.S2_at | −2.53 | Myosin light chain [xl] | = | BM172707 |
| Xl.11094.1.S1_at | −3.28 | Skeletal muscle. calcium channel α subunit [xl] | = | CD100310 |
| Xl.23777.1.S1_at | −3.63 | Troponin T [hs] | + | BG730694 |
| Xl.1251.1.S1_at | −3.71 | Na.K+-ATPase γ subunit [xl] | = | Y11587.1 |
| Xl.17369.1.S1_at | −5.29 | μ/m-Calpain large subunit [xl] | = | AB061521.1 |
| Striated muscle-cardiac | ||||
| Xl.1407.1.S1_at | 3.43 | K+ channel β2 subunit [xl] | = | BJ086795 |
| Xl.1120.1.S1_at | 3.33 | Acetylcholine receptor γ subunit [xl] | = | BF231684 |
| Xl.15700.1.S1_at | −3.69 | Calsequestrin 2 [xl] | = | BC041283.1 |
| Xl.5188.1.A2_at | −4.64 | Cardiac myosin heavy chain [xl] | = | BG730874 |
An additional exhaustive list of other affected tissue-specific transcripts is given as Supplemental Table 1. Clone ID corresponds to the position of the gene on the Affymetrix chip. BLAST best hit indicates the clone identity (xl, X. laevis; hs, Homo sapiens; mm, M. musculus). The fold of change indicates the relative level of expression of each gene in xFxr1.MO double-injected embryos relative to wild-type embryos. Only genes displaying a 2.5-fold, or greater, changes were considered. =, Xenopus gene; +, weak similarity; ++, moderate similarity; and +++, strong similarity.