Figure 3.

Autonomic neurophysiology in EAAN. Membrane potential of SMG neurons recorded using intracellular sharp microelectrodes. All SMG neurons from mice injected with rabbit control IgG (Control) showed spontaneous f-EPSPs and APs. When the attached segment of colon is distended (colonic intraluminal pressure is shown in the top trace), the frequency of f-EPSPs and APs increased. SMG neurons from mice injected 2-3 d earlier with rabbit ganglionic AChRIgG (EAAN) had normal resting membrane potential, but many showed no spontaneous or distension-evoked f-EPSPs or APs. Distention of the colon did elicit a slow membrane depolarization. In SMG neurons tested 27 d after injecting ganglionic AChRIgG (Recovery), spontaneous and distension-evoked f-EPSPs were present, and the frequencies of f-EPSPs and APs were higher than in control ganglia.