Fig. 4.

pPRIME vectors can accommodate a variety of reporter genes. (A) Schematic representation of pPRIME–CMV derivatives. For more detailed vector information see Fig. 6. PGK, phosphoglycerate kinase promoter; IRES, internal ribosome entry site. (B) U2OS cells were transduced with the indicated lentiviruses (MOI <0.4, uninfected cells served as control). GFP-, dsRed-, and LNGFR-expressing cells were FACS-sorted 4 days after infection, and Neo-expressing cells (CMV, CMV-NEO, and CMV-GIN) were selected with 500 μg/ml G418 for 1 week. Drug selection was withdrawn 1.5 days before harvesting the cells. Whole-cell extracts were analyzed by immunoblotting for the indicated proteins.