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VLP-2D9 binding affinity determination via the Langmuir absorption and Hill models
| [2D9] immobilized | Langmuir absorption model | Hill model | |||
|---|---|---|---|---|---|
| K/nM | r | K/nM | n | r | |
| 0.542 ng/mm2 | 7 ± 2 | 0.994 | 5 ± 2 | 1.2 ± 0.2 | 0.995 |
| 0.989 ng/mm2 | 11 ± 2 | 0.9981 | 6.5 ± 0.7 | 1.16 ± 0.05 | 0.9996 |
| 1.918 ng/mm2 | No fit | 9 ± 2 | 2.4 ± 0.5 | 0.9934 | |
n: stoichiometry of binding, r: correlation coefficient/quality of fit, K: Kd in nM.
