Table 4.

Summary of mutations identified in GI.2 P domain for 2D9 binding analysis

Construct	Mutated loop sequence	Position in VP60	No of amino acid changes
L1´	SASYPGNNATNVLQF	301–315 (L1)	7
L2´	NAGSAIDNPISQVAPDGFPDMSFVPFNGPGIPAA	319–352 (L2)	8
L3´	WNSNSGAPNVTTVQA	361–375 (L3)	3
L4´	TGAPGNLQ	382–389 (L4)	2
L5´	IYAVVTGTAQNPA	404–416 (L5)	7
L1´L4´	SASYPGNNATNVLQF/TGAPGNLQ	301–315/382–389	9
L4´L5´	TGAPGNLQ/IYAVVTGTAQNPA	382–389/404–416	9
L1´L4´L5´	SASYPGNNATNVLQF/TGAPGNLQ IYAVVTGTAQNPA	301–315/382–389/404–416	16
N387D	TGAPSDPQ	387	1
N387A	TGAPSAPQ	387	1
AT414-15	IYGVATGINQTAA	414–415	2

Underlined residues indicate amino acid changes with respect to wild type GI.2.