Table 1.
d-2-Hydroxyglutarate Dehydrogenase Activity in HEK-293 Cells Overexpressing the Wild-Type or Mutated Human Enzyme[Note]
| Transfectedd-2-Hydroxyglutarate Dehydrogenase | Activitya(pM/min/mg protein) |
| Wild type | 2.00 ± .3 |
| Val444Ala mutant | .5 ± .1 |
| Ile147Ser mutant | 0 |
Note.— Transient transfections were performed as described elsewhere (Achouri et al. 2004). The nucleic acid sequences of the mutated plasmids were confirmed by sequence analysis. The enzymatic activity was assayed by use of the radiochemical assay in the presence of 2 μM d-2-hydroxyglutarate without added Co2+.
a
Activities are expressed as the activity observed after subtraction of the value in nontransfected HEK cells (0.16 ± 0.02 pM/min/mg protein). Data are mean ± SEM of three or four transfections.