Fig 2. Proteomic changes in Naegleria fowleri co-cultured with bacterial or mammalian cells and amoebae isolated from infected mice.

(A) Venn diagram showing the overlap of upregulated proteins between short-term (6 h) Naegleria co-cultures with Klebsiella aerogenes or human fibrosarcoma cells HT1080, long-term (5 passages) co-culture with HT1080 cells and amoebae isolated from the brain of infected mice. (B) Venn diagram showing the overlap of 18 upregulated proteins between Naegleria long-term co-culture with human fibrosarcoma cells HT1080 and brain isolated amoebae. These proteins, listed in the table, include (marked in red) proteins involved in sulfur and lipid metabolism and lysozyme, which is discussed in the text as a potential novel virulence factor. Proteins were manually annotated by HHpred [70].