Table 1.

PCR Primers Used for Mutation Detection in the PDCD10 Gene

	Oligonucleotide Sequence
Exon(s)	Forward	Reverse	Product Size(bp)
1a	GAGTCCCCATAAGCCTCT	TTCCTCCTCCCTTTTCTCT	552
2a	CCCCTGCTTTGTAAGTAAGA	TAATCCCTCGGTTTCCTC	300
3a	AAAACTGGAAATGGAAGACA	ATTGCTTGGACCTGGAAG	453
4a	CCAACTAGGTTTGCTTTCAC	GCACCGATAAGAGTTCATTC	478
5a	CTCAGAAATGTGCTTTTTCC	AACAGGCATAAGATGGCTAA	238
6a	TCATGACACCTGCTTTACAA	ACAGTAGGGAAGGAAGATCC	359
7a	GCTAATGAATTCTGCTTTGC	GAAACCAAACGCCATAAAGT	441
8a	GAAGTGATTGCGCTTAACAT	CAACTAGGCATAAACCAACATC	280
9a	TAAAGTGCATCCCATATCCT	TGGCTAGATTAGCAACCATT	358
10a	ATTACCAGTCAGAACCACCA	CCTTCAGGAGGGACTGATA	400
4–10b	GTGAATGAAGATTCCTCTGC	CCTTCAGGAGGGACTGATA	802

^aGenomic DNA amplification was performed for this exon with the use of PCR primers; mutation detection was done by direct sequencing. Coding exons include exons 4–10.

^bcDNA studies were performed for these exons by the use of RT-PCR primers.