Sequence comparison of vertebrate PEX26 orthologs. Alignment of human (Hs), mouse (Mm), T. nigroviridis (Tn), and D. rerio (Dr) PEX26 amino acid sequence. HsPEX26 is 73%, 32%, and 31% identical to mouse, T. nigriviridis, and D. rerio PEX26, respectively. Amino acids identical across all four species are highlighted in black; those with a plurality are in gray. The positions of PEX26 missense mutations identified in this study are indicated. The solid line marks the segment of human PEX26 encoded by the alternatively spliced exon 5; the dashed line indicates the predicted transmembrane domain.