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. 2005 Apr 27;76(6):987–1007. doi: 10.1086/430637

Figure 6.

Figure 6

PEX26 segments aa 29–174 and 2–163, sufficient for PEX6 binding, as detected in a nuclear mislocalization assay. We transfected fibroblasts lacking peroxisomes due to an inherited deficiency of PEX3 (PBD400) with pcDNA3–3× mycPEX6 and either nonrecombinant pcDNA3 vector alone or recombinant pcDNA3 expressing N-terminal HA-tagged, nls-PEX26 fragments comprising the indicated segments of PEX26, and we analyzed them by indirect immunofluorescence with anti-HA (Texas Red), anti-myc (FITC) antibodies, and DAPI counterstaining, to show nuclei. The bottom two rows show expression of PEX26 segments sufficient to bind PEX6 but with the indicated missense mutations.