Table 2.
Cryo-EM data collection and data refinement data
| Cytochrome bc/JNJ-2901 | |
|---|---|
| Data collection | |
| Microscope | Titan Krios |
| Voltage (kV) | 300 |
| Magnification | ×105,000 |
| Detector—GIF | Gatan K3—Bioquantum |
| Data collection software | EPU |
| Electron exposure (e–/Å2) | 50 |
| Defocus range (μm) | 0.8–2.5 |
| Pixel size (Å) | 0.836 |
| Data processing | |
| Software | Relion 4 |
| Number of micrographs | 4522 |
| Final number of particles | 42,116 |
| Symmetry imposed | C1 |
| Map resolution (Å) | 3.1 |
| FSC threshold | 0.143 |
| Model refinement | |
| Software | Phenix 1.18.2 |
| Map correlation coefficient | 0.83 |
| Model composition | |
| Number of chains | 22 |
| Non-hydrogen atoms | 43,307 |
| Protein residues | 5206 |
| Ligands | 59 |
| Validation | |
| R.M.S. deviations | |
| Bond lengths (Å) | 0.004 |
| Bond angles (°) | 0.814 |
| Ramachandran plot | |
| Favored (%) | 96.6 |
| Allowed (%) | 3.4 |
| Disallowed (%) | 0 |
| Rotamer outliers (%) | 0 |
| Data availability | |
| EMDB entry | EMD-51689 |
| PDB entry | 9GY6 |