Table 2.
Table summarizing the affinity parameters of the interaction between the tau[369−381] C-terminal peptide and VHHs E2-2 or H3-2
| VHH | Kinetic analysis | Steady-state analysis | |||
|---|---|---|---|---|---|
| kon (M−1.s−1)*10+2 | koff (s−1)*10-3 | KD (nM) | KD (nM) | SR | |
| E2-2 | 1540 ± 271 | 124 ± 13 | 820 ± 100 | 578 ± 213 | 0.45 |
| H3-2 | 1918 ± 359 | 71 ± 2 | 280 ± 70 | 297 ± 84 | 0.41 |
Kinetic kon and koff, and KD values are included in the table. For the steady-state analysis, KD were determined using the one-site specific binding model. The stoichiometric ratio (SR) was calculated from the ratio of the experimental Rmax to the theoretical Rmax.