Table 2. Multivariate models examining the associations between NfL levels and clinical variables.

		sNfL			cNfL
Variables		Estimate*	95% CI	P value	Estimate*	95% CI	P value
Model 1
Age at diagnosis		0.02	−0.04 to 0.08	0.510	0.01	−0.06 to 0.09	0.717
Onset site	Bulbar	−0.04	−0.13 to 0.05	0.380	−0.01	−0.13 to 0.11	0.811
Revised ElEscorial criteria	Definite or probable	0.10	0.03 to 0.17	0.007	0.03	−0.06 to 0.12	0.448
Disease progression rate	ΔFS	0.09	0.04 to 0.15	0.002	0.09	0.02 to 0.16	0.012
Model 2
Age at diagnosis		0.02	−0.04 to 0.07	0.572	0.01	−0.06 to 0.08	0.771
Onset site	Bulbar	−0.06	−0.15 to 0.04	0.221	−0.03	−0.16 to 0.09	0.568
Revised ElEscorial criteria	Definite or probable	0.10	0.03 to 0.17	0.005	0.04	−0.05 to 0.13	0.346
Disease progression rate	Δ%FVC	0.03	0.01 to 0.05	0.001	0.03	0.01 to 0.05	0.010
Model 3
Age at diagnosis		0.01	−0.05 to 0.07	0.681	0.01	−0.06 to 0.08	0.817
Onset site	Bulbar	−0.04	−0.13 to 0.06	0.460	−0.01	−0.12 to 0.11	0.914
Revised ElEscorial criteria	Definite or probable	0.10	0.03 to 0.18	0.008	0.04	−0.05 to 0.13	0.351
Disease progression rate	FVC-DiP	−0.05	−0.08 to −0.02	0.004	−0.06	−0.09 to −0.02	0.004

^*pPer 10-year-year increase for age, per 1-point increase for the ΔFS and Δ%FVC, and per 10-point increase for the FVC-DiP score.

cNfL, cerebrospinal fluid neurofilament light chain; %FVC, percentage of predicted forced vital capacity; FVC-DiP, FVC decline pattern scalesNfL, serum NfL; ΔFS, rate of decline in the Amyotrophic Lateral Sclerosis Functional Rating Scale Revised; Δ%FVC, rate of decline in the %FVC