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. 2025 Mar 31;13:e19236. doi: 10.7717/peerj.19236

Figure 5. Tumor cells acquire gDNA from T cells.

Figure 5

(A) Flow cytometry assay of MDAMB231 cells co-cultured with EdU-labeled CD3+ T cells. (B) Flow cytometry analysis of MDAMB231 cells co-cultured with H2B-GFP CD3+ T cells. (C) Flow cytometry plot showing four populations of CD45- H2B-GFP- (lower left gate), CD45+ H2B-GFP- (upper left gate), CD45+ H2B-GFP+ (upper right gate), CD45- GFPH2B+ (lower right gate) MDAMB231 cells. (D) Visualization of trogosomes within a MDAMB231 cell containing CD45 (magenta), Hoechst dye (blue), and GFP-H2B (Green). (E) Single channel images of single trogosome containing GFP-H2B, Hoechst dye, and CD45.