Figure 3.

CD47, but not TSP1 and SIRPα, protein is found in human islet lysate. Lysates of islet preparations from donors without diabetes (n = 5, Hu 1186, Hu 1199, Hu 1228, Hu 1229, and Hu 1232) underwent separation by gel electrophoresis and protein expression was characterized by Western blot. Representative Western blot showing CD47, TSP1, and SIRPα expression (A). Densitometry analysis of CD47, TSP1, and SIRPα expression relative to β-actin (B). Each dot represents a donor. Data are presented as the means ± SD. Significance was determined using one-way ANOVA for multiple comparisons. SIRPα, signal regulatory protein α; TSP1, thrombospondin-1.