Figure 4.

CD47 resides on the surface of islet cells. Islets from donors without diabetes (n = 3, Hu 1232, Hu 1237, and Hu 1239) were dissociated and stained with fluorochrome-conjugated anti-CD47 or anti-SIRPα antibodies and analyzed by flow cytometry. Gating strategy for single live cells (A). Representative analysis of CD47- (B) and SIRPα-positive cells (C) after sequential gating from A. Positive thresholds were set according to isotype controls. Quantification of the % CD47- and SIRPα-positive cells (D). Each dot represents one donor. Data are presented as the means ± SD. Significance was determined using paired t tests. **P ≤ 0.01. SIRPα, signal regulatory protein α.