Table 2.
Antibody information
| Primary antibody (Ref.) | Assay | Company | Cat. No. | Animal Source | Dilution |
|---|---|---|---|---|---|
| Anti-Insulin (27) | IF | Abcam | ab195956 | Guinea pig | 1:200 |
| Anti-CD47* | IF | Invitrogen | PA5-116827 | Rabbit | 1:200 |
| Anti-TSP1 (28) | IF | Invitrogen | MA5-133398 | Mouse | 1:100 |
| Anti-SIRPα (29) | IF | Abcam | ab191419 | Rabbit | 1:1,000 |
| Anti-CD47 | WB | Invitrogen | PA5-116827 | Rabbit | 1:1,000 |
| Anti-TSP1 | WB | Invitrogen | MA5-133398 | Mouse | 1:250 |
| Anti-SIRPα | WB | Cell Signaling | 13379 | Rabbit | 1:1,000 |
| Anti-PD-L1 | WB | Cell Signaling | 13684S | Rabbit | 1:1,000 |
| Anti-β-actin | WB | Cell Signaling | 3700 | Mouse | 1:1,000 |
| Anti-CD47 BV605 | FC | Biolegend | 323120 | Mouse | 1:20 |
| Anti-SIRPα PE | FC | Biolegend | 372103 | Mouse | 1:20 |
| Secondary antibody | |||||
| Anti-Guinea pig Alexa 488 | IF | Jackson | 706-545-148 | Donkey | 1:200 |
| Anti-Mouse Alexa 647 | IF | Jackson | 715-606-150 | Donkey | 1:200 |
| Anti-Rabbit Alexa 647 | IF | Jackson | 715-606-152 | Donkey | 1:200 |
| Anti-Mouse IgG HRP | WB | Cytiva | N931 | Sheep | 1:3,000 |
| Anti-Rabbit IgG HRP | WB | Cytiva | N934 | Donkey | 1:3,000 |
FC, flow cytometry; IF, immunofluorescent staining; sc-RNAseq, single-cell RNA sequencing; WB, Western blot. *This antibody was validated by using U2 OS cell (IF), mouse testis tissue (IHC), and Western blot from manufacturer: https://www.thermofisher.com/antibody/product/CD47-Antibody-Polyclonal/PA5-116827.