Fig. 2. K116 directly targeted STAT3 in breast cancer cells. (a) Scheme of the CETSA-WB experiment. (b) CETSA-WB experiment to further confirm the interaction between K116 (5 μM, 20 μM, and 25 μM) and STAT3. (c) The thermal stabilities of STAT3 with or without K116 (25 μM). (d) CETSA-WB experiment to further confirm the interaction between K116 (25 μM) and pY705STAT3, Akt, STAT1, and STAT5. (e) The thermal stabilities of pY705STAT3 with or without K116 (25 μM). (f) Docking model of K116 with the CCD. The crystal structure of STAT3 (PDB ID: 3CWG) was downloaded from the PDB. (g) Sequence alignment of the coiled-coil domain (CCD) of STAT3, STAT1, and STAT5.
