| Method |
Vapor diffusion, sitting drop |
| Plate type |
SWISSCI MRC2 |
| Temperature (K) |
287 |
| Protein concentration (mg ml−1) |
26.2 |
| Buffer composition of protein solution |
25 mM HEPES pH 7.0, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% sodium azide |
| Composition of reservoir solution |
Microlytics MCSG1 screen condition C9: 32%(w/v) PEG 4000, 800 mM lithium chloride, 100 mM Tris–HCl pH 8.50. The crystal was soaked for 6 h in reservoir with 5 mM SAH and cryoprotectant (soaking buffer with 20% ethylene glycol). |
| Volume and ratio of drop |
0.4 µl protein solution + 0.4 µl reservoir solution |
| Volume of reservoir (µl) |
80 |
