Table 1.
In vitro forward mu tation assay error results for HIV-RT utilizing either natural or gamma-modified dNTP substrates
| Mutational class | dNTPs | ANS-dNTPs | |||
|---|---|---|---|---|---|
| Number of mutants | Mutational frequency (× 10−4) | Number of mutants | Mutational frequency (× 10−4) | ||
| Single-base substitutions | 178 | 124 | 26 | 20 | |
| Single-base frameshifts | 33 | 23 | 5 | 4 | |
| Multiple mutations | 38 | 5 | |||
| Mutation: From→To | Fold reduced w/ANS | ||||
| T_C | 113 | 93 | 10 | 9 | 10 |
| G_T | 26 | 21 | 9 | 8 | 3 |
| A_T | 2 | 2 | 2 | 2 | 1 |
| A_C | 3 | 2 | 1 | 1 | 2 |
| C_T | 6 | 5 | 2 | 2 | 3 |
| G_C | 4 | 3 | 1 | 1 | 3 |
| A_G | 4 | 3 | 1 | 1 | 3 |
| G_A | 10 | 8 | 0 | CD | >8 |
| T_G | 3 | 2 | 0 | CD | >3 |
| C_A | 5 | 4 | 0 | CD | >4 |
| T_A | 2 | 2 | 0 | CD | >2 |
| C_G | 0 | CD | 0 | CD | CD |
The results are the sum of two independent reactions per substrate at a concentration of 500 µM each. If a clone contained more than one mutation, each mutation was counted as an individual event. ‘Multiple mutants’ indicates the number of phage containing more than one mutation. CD: cannot be determined.