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. 2025 Jan 20;14:110. [Version 1] doi: 10.12688/f1000research.158910.1

Figure 13. Setting up and running the humanised in vitro thrombosis model.

Figure 13.

(A) Transfer the TEIL onto the surface of the microfluidic chip (B) Place the cutter tool onto the TEIL with the sharp edge pointing downwards (C) Pull the nanofibre frame up whilst producing downward pressure through the cutting tool to sever the nanofibers (D) Place the TEML on top of the TEIL using the guide marks on the microfluidic chip (E) Place the microfluidic chip onto the positioner in the mechanical injury press (F) puncture TEIL by lowering the needle block of the mechanical injury press onto the TEAC (G) remove the injured TEAC from the mechanical injury press (H) Screw the compression bracket onto the microfluidic chip to produce the completed thrombus-on-a-chip model (I) Attaching tubing to the microfluidic chip and connect to a syringe pump containing whole human blood with CFSE-labelled platelets (J) Place under a fluorescent microscopy and initiate perfusion at the desired flow parameters. Monitor thrombus formation in real time using the fluorescence microscope whilst collecting post-flow blood samples into eppendorfs containing ACD. Figure created with BioRender.com.