Product	Company	Notes
Human Coronary Artery Smooth Muscle cells (HCASMCs)	Fisher Scientific (Loughborough, UK)	Cells for Tissue Engineered Medial Layer Primary human cells must be stored, used and disposed of in premises with a human tissue authority license Used up to Passage 5
Human umbilical vein endothelial cells (HUVECs)	Fisher Scientific (Loughborough, UK)	Cells for Tissue Engineered Intimal Layer Primary human cells must be stored, used and disposed of in premises with a human tissue authority license Used up to Passage 5
Human Vascular Smooth Muscle Cell Medium (previously known as Medium 231)	Fisher Scientific (Loughborough, UK)	Media for HCASMCs culture Must be kept sterile
Human Large Vessel Endothelial Cell Basal Medium (previously known as Medium 200)	Fisher Scientific (Loughborough, UK)	Media for HUVEC culture Must be kept sterile
Smooth Muscle Growth Supplement (SMGS)	Fisher Scientific (Loughborough, UK)	Media for HCASMC culture Must be kept sterile
Low Serum Growth Supplement	Fisher Scientific (Loughborough, UK)	Growth Supplement for HUVEC culture Must be kept sterile
α-MEM medium powder	Fisher Scientific (Loughborough, UK)	Component of medial layer hydrogel Used to produce a sterile 10x stock solution
CellTrace TM Violet Proliferation Kit	Fisher Scientific (Loughborough, UK)	Fluorescent cell label used to track culture of HUVEC growth on nanofibers Must be kept sterile
Corning High Concentration Rat Tail Type I Collagen	Fisher Scientific (Loughborough, UK)	Component of medial layer hydrogel Must be kept sterile
Whatman Qualitative Filter paper	Scientific Laboratory Supplies (Nottingham, UK).	Used to produce filter paper frames Autoclave to sterilise prior to use
Lonza Penicillin-Streptomycin-Amphotericin B	Scientific Laboratory Supplies (Nottingham, UK).	Prevents infection of primary cell cultures Must be kept sterile
L-Ascorbic Acid	Sigma Aldrich (Gillingham, UK)	Supports enhanced thrombogenicity of medial layer by supporting HCASMC collagen production and tissue factor activity Must be kept sterile
DETA-NONOate	Cambridge Bioscience (Cambridge, UK).	Nitric Oxide donor to inhibit medial layer gel contraction Must be kept sterile
Human Fibronectin Protein, CF	R&D Systems (Abingdon, UK)	PLA nanofibre coating to improve HUVEC adherence and proliferation Must be kept sterile