Table 3. Specification of primers and probes used in Trypanozoon-RT-qPCR multiplex nucleic acid detection.

Name	Description	Oligonucleotide sequence (5’>3’)	Label	Reference
TBRF	Forward primer for TBR sequences	CGC AGT TAA CGC TAT TAT ACA	none	Van Reet et al., 2021
177 T-R	Reverse primer specific for 177-bp TBR group	GGA CCA TTA AAT AGC TTT GTT G	none	Van Reet et al., 2021
177 T-P	Probe specific for 177-bp TBR group	TGC CAT ATT AAT TAC AAG TGT GC	FAM, BHQplus-1	Van Reet et al., 2021
18S2-F	Forward primer for 18S rRNA	CCA ATC GGA CGC TCT CTT T	none	N’Djetchi MK, et al. 2024
18S2-R	Reverse primer for 18S rRNA	GTG GAG GCG TTG GTT CTA AT	none	N’Djetchi MK, et al. 2024
q18S2-P	Probe for 18S rRNA	TTG TGT TTA CGC ACT TGT CGT GGC	LGC Orange 560, BHQplus-1	N’Djetchi MK, et al. 2024
RP-F	Human RNAse P forward primer	AGA TTT GGA CCT GCG AGC G	none	Lu et al., 2020
RP-R	Human RNAse P reverse primer	GAG CGG CTG TCT CCA CAA GT	none	Lu et al., 2020
RP-P	Human RNAse P probe	TTC TGA CCT GAA GGC TCT GCG CG	Cy5, BHQplus-2	Lu et al., 2020

The role, oligonucleotide sequence, presence of a label and corresponding reference are listed for primers and probes used for nucleic acid detection by Trypanozoon-RT-qPCR multiplex in the STROGHAT study.