Figure 4.

Vascularization Defects in the tgh Mutants.

(A) and (B) Cleared cotyledons and leaf of wild-type plants.

(C) GUS staining of AtHB-8:GUS in cotyledons of 5-d-old Arabidopsis seedlings clearly delineates the vascular system.

(D) and (E) Cleared cotyledons and leaf of the tgh-1 mutant reveal defects in vascularization. Arrowheads mark unconnected vascular strands.

(F) GUS staining of AtHB-8:GUS in the cotyledons of tgh-1 seedlings delineates the interrupted vascular system observed in these mutants. Arrowheads mark unconnected vascular strands.

(G) to (L) GUS expression in embryos (G), cotyledon (H), leaf (I), young inflorescence (K), and the pistil (L) of transgenic lines expressing the reporter GUS under control of a 1021-bp TGH promoter fragment.