Figure 5.

Auxin Response and Auxin Transport Are Not Affected in the tgh-1 Mutant.

(A) Auxin-induced gene expression of the genes GH3, IAA19, and SAUR10 is not impaired in tgh-1 mutants compared with the wild type. Three-week-old plants were treated for 2 h with the synthetic auxin 2,4-D and subsequently analyzed by reverse transcription followed by 28 PCR amplification cycles.

(B) TGH gene expression is obviously unaltered in 7-d-old bdl, mp, and axr6-2 mutant seedlings as determined by RT-PCR analysis. ACTIN served as an input control for all experiments. Number of PCR amplification cycles is indicated in parentheses.

(C) to (F) Mutants defective in TGH and PIN1 show additive phenotypes. Shown are 4-week-old wild-type (C), tgh-1 (D), pin1-3 (E), and pin1-3 tgh-1 double mutant (F) plants as well as flowers (insets). Bars = 1.5 cm.