Table 5. Intestinal histomorphology of fish exposed to different experimental treatments for 60 days.
Variable | CG | SB1 | SB2 | SB3 | p value |
---|---|---|---|---|---|
Anterior part | |||||
Villi length (μm) | 151.487 ± 15.48a | 227.611 ± 17.604b | 376.646 ± 28.711c | 286.525 ± 11.085d | <0.0001 |
Villi width (μm) | 112.108 ± 20.842a | 114.84 ± 7.845a | 86.42 ± 30.315a | 100.855 ± 3.95a | 0.3114 |
Goblet cell/mm2 | 32 ± 3.606a | 40 ± 3.606a | 70.333 ± 5.508b | 54.667 ± 6.506c | <0.0001 |
The inter-villi space (μm) | 104.943 ± 14.55a | 80.878 ± 6.64ac | 42.490 ± 9.29b | 52.941 ± 13.692bc | 0.0029 |
Middle part | |||||
Villi length (μm) | 373.497 ±24.584a | 491.627 ±43.98ac | 738.84 ±39.57b | 593.617 ± 133.102bc |
0.0018 |
Villi width (μm) | 88.165 ±8.685a | 95.728 ±7.280a | 65.887 ±9.523b | 81.340 ±7.387ab | 0.0122 |
Goblet cell/mm2 | 50.667 ±3.055a | 64.334 ±3.215b | 90.00 ±4.001c | 77.00 ±7.00d | <0.0001 |
The inter-villi space (μm) | 33.357 ±3.571a | 23.160 ±5.784b | 8.823 ±0.577c | 12.093 ±2.303c | 0.0001 |
Posterior part | |||||
Villi length (μm) | 69.780 ±11.628a | 89.27 ±8.552a | 197.925 ±10.616b | 123.389 ±5.384c | <0.0001 |
Villi width (μm) | 125.567 ±45.946a | 87.454 ±10.912a | 87.972 ±10.454a | 106.678 ±19.90a | 0.3013 |
Goblet cell/mm2 | 17.33 ±3.055a | 25.33 ±2.516ac | 39.33 ±3.511b | 33.667 ±3.785bc | 0.0002 |
The inter-villi space (μm) | 123.111 ±33.495a | 99.507 ±22.469ac | 37.59 ±10.55b | 56.294 ±17.649bc | 0.0061 |
Note: Means ± SD within the same row with different superscripts are significantly different (p< 0.05). Different superscript letters (a, b, c, d) indicate significant differences between different groups: CG, the fish received basal diet; Syrena Boost group 1 (SB1), fish received basal diet containing 0.1 g kg–1 Syrena Boost; Syrena Boost group 2 (SB2), fish received basal diet containing 0.2 g kg–1 Syrena Boost; Syrena Boost group 3 (SB3), fish received basal diet containing 0.4 g kg–1 Syrena Boost.