Table 1.
List of primers used to amplify and sequence the DNA barcoding regions COI and rRNA genes.
| Primers | Primer sequence | Source |
|---|---|---|
| COI | ||
| LCO1490_PH (forward) | 5’-TTTCWACTAATCATARGGATATTGG-3’ | modified from LCO1490aphonopelma (Hamilton et al. 2011) |
| HCO2198_PH (reverse) | 5’-TAAACCTCCGGATGWCCAAAAAAYCA-3’ | modified from dgHCO-2198 (Meyer 2003) |
| C1-J-2123_PH (forward) | 5’- GATCGAAATTTTAATACTTCKTTYTTTGA-3’ | modified from C1-J-2123 (Vidergar et al. 2014) |
| C1-N-2776_PH (reverse) | 5’-GGATAATCAGAATATCGTCGAGGTATTCCAT-3’ | modified from C1-N-2776 (Hedin and Maddison 2001) |
| 12S–tRNA-Val–16S | ||
| 12SUST_PHTarantula (forward) | 5’-CGTCACCCTCGTCCAAAGAT-3’ | this study |
| 16SUST_PHTarantula (reverse) | 5’-CGATAGGGTCTTGTCGTCCC-3’ | this study |