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Sphingosine alters the permeability of the outer bacterial membrane. P. aeruginosa ATCC 27853 or 762 were incubated with 0.1 µM sphingosine or left untreated for 15 min in H/S followed by 2 h incubation in 10 mL TSB supplemented with 0.5% sodium dodecyl sulfate (SDS) and 0.5 mM EDTA. 0.5% SDS alone was without effect. Aliquots of the bacteria were plated on TSA, and CFUs were counted after o/n growth. Shown are the mean ± SD of five independent experiments; ***P <0.001, ANOVA.
