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. 2025 Mar 26;18:1552819. doi: 10.3389/fnmol.2025.1552819

Figure 2.

Figure 2

Astrocyte differentiation and identification of cellular characteristics. (A) The cellular morphological characteristics of hiPSCs, P0 NPCs, and P1 NPCs during the cultivation process (scale bar = 250 μm). (B) HiPSCs were identified by OCT4 cell marker (scale bar = 50 μm). (C) All NPCs expressed Nestin and SOX2 cell markers (scale bar = 50 μm). (D) The cell morphological changes were observed during this 30-day astrocyte differentiation period. Bright-field microscopy images of astrocytes at day 1, day 10, and day 30 during differentiation (scale bar = 250 μm). (E) All astrocytes were positive for S100B expression (scale bar = 100 μm). (F) All astrocytes were positive for GFAP expression (scale bar = 100 μm).