Figure 6.

Immunocytochemical detection of DS hiPSC-derived reactive astrocyte markers. Three pairs of cell lines (C2 vs. DS2, C4 vs. DS3, and C5 vs. DS4) were used to perform ICC. Fluorescence micrographs of EAAT2, S100A10 and S100B staining and statistical analysis across different groups: Con group (Disomic astrocytes), Con + X5050 group (Disomic astrocytes with X5050 treatment), DS + Li group (Trisomic astrocytes with lithium carbonate treatment), DS + Li + X5050 (Trisomic astrocytes with both lithium carbonate and X5050 treatment). The one-way ANOVA was employed to assess the differences between groups. ns, not significant; *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001, ****p-value < 0.0001. Scale bar = 100 μm.