Table 2.

List of primer sequences for RT-qPCR.

Gene symbols	Forward primers (5′ → 3′)	GC%	Reverse primers (5′ → 3′)	GC%	Annealing temperature (°C)	Amplicon size (bp)
GFAP	CAGATTCGAGGGGGCAAA	55.56	TGAGAGGCAGGCAGCTA	58.82	60	149
EAAT1	ACATGAAGGAACAGGGGCAG	55.00	CACGGGGGCATACCACATTA	55.00	60	98
EAAT2	TGCCAACAGAGGACATCAGCCT	54.55	CAGCTCAGACTTGGAGAGGTGA	54.55	60	128
HMBS	GCCGTGCATACAGCTATGAAG	52.38	TTACGAGCAGTGATGCCTACC	52.38	60	179
IL10	GGCACCCAGTCTGAGAACAG	60.00	TGGCAACCCAGGTAACCCTTA	52.38	60	176
IL1B	GCTCTGGGATTCTCTTCAGCC	57.14	CAAGTCATCCTCATTGCCACTGT	47.83	60	118
PSMB2	GAGGGCAGTGGAACTCCTTAG	57.14	GATGTTAGGAGCCCTGTTTGG	52.38	60	149
REST	GAGAACGCCCATATAAATGTG	42.86	CACATAACTGCACTGATCAC	45.00	60	172
S100A10	CGCCGCACGTACTAAGGAA	57.89	GTGTGGTCCGTTGAAGCCTTG	57.14	60	118
S100B	GTGGCCCTCATCGACGTTTT	55.00	ACCTCCTGCTCTTTGATTTCCTCT	45.83	60	134
TNF-α	GCCCATGTTGTAGCAAACC	52.63	TCTGGTAGGAGACGGCGAT	57.89	60	226
TGF-β	TTGAGGGCTTTCGCCTTAGC	55.00	CGGTAGTGAACCCGTTGATG	55.00	60	84

F, Forward Primer; R, Reverse Primer; GFAP, Glial fibrillary acidic protein; EAAT1, Excitatory amino acid transporter 1; EAAT2, Excitatory amino acid transporter 2; HMBS, Hydroxymethylbilane synthase; IL-10, Interleukin 10; IL-1B, Interleukin-1 beta; PSMB2, Proteasome 20S subunit beta 2; REST, RE1 silencing transcription factor; S100A10, S100 calcium-binding protein A10; S100B, S100 calcium-binding protein B; TNF-α, Tumour necrosis factor; TGF-β, Transforming growth factor beta.