Figure 2.

AAV-DTA-induced apoptosis of SGN cells

(A) The injected WT control (7 dpi) retains a healthy population of SGN in the Rosenthal’s canal (RC) with negligible expression of cleaved caspase-3. Cleaved caspase-3 expression (white arrowheads) is upregulated in PV^Cre mice both 3 and 7 days after AAV-DTA injection. There is a noticeable degeneration of TUJ1⁺ SGN by 7 days from AAV-DTA injection. (B) AAV-DTA-injected WT control mice uniformly express cytochrome c in the cytoplasm of SGN 7 dpi. Inserts demonstrate high magnification of cytochrome⁺ SG cells; the cytoplasm (yellow arrowheads) becomes disorganized in PV^Cre mice 3 and 7 dpi.