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. 2025 Apr 9;17:124. doi: 10.1186/s13098-025-01686-9

Table 1.

Characteristics of participants in the NHANES (2007–2016) by Klotho levels

Klotho levels
Variables Q1 Q2 Q3 Q4 P-value
Participants, No. 890 890 890 890
Klotho(pg/ml) 525.9(87.7) 723.5(44.8) 897.4(57.6) 1290.7(331.4) < 0.001
Clinical parameters
Age(years) 62.8(10.0) 61.8(10.1) 61.1(9.9) 59.8(10.0) < 0.001
Gender, n (%) 0.272
 Female 424 (47.6) 416 (46.7) 452 (50.8) 445 (50.0)
 Male 466 (52.4) 474 (53.3) 438 (49.2) 445 (50.0)
Race, n (%) < 0.001
 Mexican American 184 (20.7) 170 (19.1) 195 (21.9) 178 (20.0)
 Non-Hispanic Black 217 (24.4) 187 (21.0) 173 (19.4) 253 (28.4)
 Non-Hispanic White 322 (36.2) 327 (36.7) 305 (34.3) 253 (28.4)
 Other Hispanic 92 (10.3) 119 (13.4) 119 (13.4) 134 (15.1)
 Other Race 75 (8.4) 87 (9.8) 98 (11.0) 72 (8.1)
PIR 2.2 (1.5) 2.3 (1.5) 2.2 (1.5) 2.3 (1.6) 0.970
Marital status, n (%) 0.971
 Divorced/ Separated 186 (20.9) 181 (20.3) 184 (20.7) 173 (19.4)
 Married/ Living with partner 547 (61.5) 555 (62.4) 547 (61.5) 549 (61.7)
 Never married/ Widowed 157 (17.6) 154 (17.3) 159 (17.9) 168 (18.9)
Smoking, n (%) 0.003
 Never smoker 405 (45.5) 393 (44.1) 467 (52.5) 457 (51.3)
 Former smoker 321 (36.1) 337 (37.9) 282 (31.7) 278 (31.2)
 Current smoker 164 (18.4) 160 (18.0) 141 (15.8) 155 (17.5)
Alcohol status, n (%) 0.566
 Never 148 (16.6) 163 (18.3) 179 (20.1) 163 (18.3)
 Former 278 (31.2) 260 (29.2) 247 (27.8) 260 (29.2)
 Current 464 (52.2) 467 (52.5) 464 (52.1) 467 (52.5)
Vital signs
BMI (kg/m2) 32.5 (7.0) 32.6 (7.2) 32.5 (7.4) 32.4(7.5) 0.984
DBP (mmHg) 67.6 (12.9) 68.7(13.1) 69.6(12.8) 70.8(12.9) < 0.001
SBP (mmHg) 130.8(19.9) 130.4(18.8) 129.3(18.4) 131.0(19.3) 0.261
Laboratory parameters
 ALT(U/L) 24.5(14.0) 25.7(13.7) 28.2(33.4) 31.4 (25.7) < 0.001
 Alb(g/L) 41.3 (3.5) 41.8(3.2) 41.8(3.3) 41.4(3.5) < 0.001
 Creatinine(umol/L) 106.1(99.0) 86.1(40.6) 81.8(53.2) 80.0 (43.6) < 0.001
 eGFR (mL/min/1.73m2) 75.1(27.0) 83.0(24.7) 86.6(22.1) 89.5(23.3) < 0.001
 TC (mg/dl) 4.7 (1.2) 4.8 (1.2) 4.9(1.1) 5.0 (1.3) < 0.001
 HDL (mmol/L) 1.3 (0.4) 1.2 (0.4) 1.2 (0.4) 1.2 (0.4) 0.561
 HbA1c (%) 6.9 (1.3) 7.1(1.6) 7.2(1.7) 7.7 (2.1) < 0.001
Comorbidities
 Hyperlipidemia, n (%) 0.223
 No 90 (10.1) 96 (10.8) 109 (12.2) 115 (12.9)
 Yes 800 (89.9) 794 (89.2) 781 (87.8) 775 (87.1)
 Stroke, n (%) 0.057
 No 795 (89.3) 823 (92.5) 822 (92.4) 818 (91.9)
 Yes 95(10.7) 67 (7.5) 68 (7.6) 72 (8.1)
 Coronary heart disease, n (%) 0.007
 No 780 (87.6) 804 (90.3) 812 (91.2) 821 (92.2)
 Yes 110 (12.4) 86 (9.7) 78 (8.8) 69 (7.8)
 Hypertension, n (%) 0.024
 No 204 (22.9) 229 (25.7) 249 (28.0) 256 (28.8)
 Yes 686 (77.1) 661 (74.3) 641 (72.0) 634 (71.2)

Abbreviations: PIR, poverty income ratio; BMI, body mass index; DBP, diastolic pressure; SBP, systolic pressure; ALT, alanine transaminase; Alb, albumin; eGFR, estimated glomerular filtration rate; TC, total cholesterol; HDL, high density lipoprotein; LDL, low density lipoprotein; HbA1c, glycohemoglobin