Figure 3.

Effect of curcumin- and emodin-derived inhibitors on the stability of c-Jun and p53. Curcumin- and emodin-derived inhibitors of CSN-associated kinases affect the stability of c-Jun and p53 in HeLa or B8 cells. HeLa cells or B8 cells were incubated for 4 h with the inhibitors emodin, resveratrol, piceatannol, BTB14431 and JFD02836. The first lane is the control without inhibitor. Proteins of cell lysates were separated by SDS-PAGE and transferred to nitrocellulose. The Western blots analysis was probed with an anti-c-Jun or an anti-p53 antibody.