Table 2.

Determination of IC50 values in an in vitro kinase assay. IC50 values (μM) for the inhibition of recombinant CK2, PKD and the purified CSN complex by the inhibitors of the curcumin-group, piceatannol as well as BTB00363, and of the emodin-group, BTB14431, JFD02836, SEW04213 and JFD03665 were determined. The two groups of inhibitors were created based on the different structures of the lead compounds (see Table 1). Kinase assays were performed as outlined in materials and methods. For comparison IC50 values of curcumin, resveratrol and emodin from earlier measurements [2] are shown.

Inhibitor	IC50 CK2 (μM)	IC50 PKD (μM)	IC50 CSN (μM)
Curcumin-group
Curcurmin	11.8	4.1	2.6 *
Resveratrol	51.0	17.6	32.1 *
Piceatannol	2.5	0.5	1.7
BTB00363	332.7	117.7	188.6
Emodin-group
Emodin	22.7	94.5	4.4 *
BTB14431	6.4	68.9	21.0
JFD02836	19.9	39.7	54.1
SEW04213	37.9	185.9	4.8
JFD03665	5.9	154.9	19.8

* [2]