Figure 1.

(A) Effect of varying laser power settings on Alexa 594 fluorescence signals. Results for the PHASE-I cDNA microarray scanned with constant PMT gain and varying laser power. Average ¹⁰log transformed fluorescence data for each gene of the dyes at varying setting (y-axis) was plotted against the initial fluorescence data (x-axis). (B) Effect of varying PMT gain settings on Alexa 594 fluorescence signals. Results for the PHASE-I cDNA microarray scanned with constant laser power and varying PMT gain. Average ¹⁰log transformed fluorescence data for each gene of the dyes at varying setting (y-axis) was plotted against the initial fluorescence data (x-axis)