Table 1.

Cross-fluorescence of tested dyes. Fluorescence of dyes at scanner settings of all dyes expressed as a percentage of fluorescence at its own settings (the latter was set to 100%).

dye	Alexa 488	Alexa 594	Cy3	Cy5
settings
Alexa 488	100.0	0.0	1.1	0.0
Alexa 594	0.9	100.0	1.3	13.0
Cy3	0.0	2.2	100.0	0.0
Cy5	0.1	0.1	0.2	100.0