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Labelling schedule for B[a]P exposed samples. Each HepG2 sample is labelled with each fluorescent dye. Rat liver samples were labelled as shown by array number 1–3, without the application of Alexa 488.
| fluorescent label array no. |
Cy3 | Cy5 | Alexa 594 | Alexa 488 |
| 1 | 0 μM | 3 μM | 10 μM | 30 μM |
| 2 | 10 μM | 0 μM | 30 μM | 3 μM |
| 3 | 3 μM | 30 μM | 0 μM | 10 μM |
| 4 | 30 μM | 10 μM | 3 μM | 0 μM |
