Table 2.

FQY, extinction coefficients (ϵ₃₄₇) and multi-exponential TCSPC fitting results for all oligonucleotides at 20°C

RNA	FQYa	ϵ347 (M−1 cm−1)		⟨τ⟩obs (ns)	w1	τ1 (ns)	w2	τ2 (ns)	w3	τ3 (ns)
pCr	0.023 ± 0.002	3700b	1.14	1.963 ± 0.001	0.991 ± 0.002	1.930 ± 0.005	0.009 ± 0.002	5.7 ± 0.6	–	–
pCpCc	0.0022 ± 0.0006	7400b	1.22	2.43 ± 0.02	0.962 ± 0.006	0.03 ± 0.01	0.035 ± 0.006	2.07 ± 0.03	0.003 ± 0.004	6.8 ± 0.3
pCA	0.050 ± 0.005	3920 ± 10	1.14	4.231 ± 0.009	0.75 ± 0.02	3.51 ± 0.05	0.25 ± 0.02	6.5 ± 0.1	–	–
ApCA	0.09 ± 0.01	2560 ± 50	1.10	8.50 ± 0.03	0.379 ± 0.004	3.27 ± 0.07	0.621 ± 0.004	11.70 ± 0.02	–	–
pCC	0.054 ± 0.006	3780 ± 60	1.07	4.52 ± 0.02	0.701 ± 0.005	3.24 ± 0.03	0.299 ± 0.005	7.52 ± 0.06	–	–
CpCC	0.080 ± 0.008	3530 ± 50	1.08	7.12 ± 0.02	0.355 ± 0.003	3.33 ± 0.06	0.645 ± 0.003	9.201 ± 0.009	–	–
pCG	0.022 ± 0.002	3370 ± 40	1.12	2.20 ± 0.02	0.864 ± 0.007	1.80 ± 0.03	0.136 ± 0.007	4.78 ± 0.08	–	–
GpCG	0.039 ± 0.004	2440 ± 90	1.14	4.02 ± 0.02	0.590 ± 0.004	2.22 ± 0.03	0.410 ± 0.004	6.63 ± 0.04	–	–
pCU	0.020 ± 0.002	3860 ± 60	1.08	2.01 ± 0.03	0.58 ± 0.03	0.98 ± 0.06	0.35 ± 0.02	2.8 ± 0.1	0.077 ± 0.009	6.2 ± 0.2
UpCU	0.031 ± 0.003	2950 ± 20	1.06	3.20 ± 0.02	0.32 ± 0.01	1.36 ± 0.05	0.64 ± 0.01	3.78 ± 0.04	0.035 ± 0.003	9.5 ± 0.3
St1	0.056 ± 0.005	3109 ± 6	1.12	6.770 ± 0.006	0.493 ± 0.007	4.46 ± 0.03	0.507 ± 0.007	9.01 ± 0.04	–	–
1:2 GGG	0.026 ± 0.002	3420 ± 70	1.18	3.225 ± 0.005	0.8682 ± 0.0003	2.074 ± 0.005	0.1318 ± 0.0003	10.81 ± 0.03	–	–
1:2 GUG	0.056 ± 0.006	3460 ± 40	1.13	6.21 ± 0.01	0.65 ± 0.01	4.72 ± 0.06	0.35 ± 0.01	8.92 ± 0.08	–	–

^aRelative FQY determined using pCr [17] as a reference.

^bBased on ϵ₃₄₇ for pC reported at https://www.glenresearch.com/reports/gr15-13.

^cShortest component omitted in calculation of ⟨τ⟩_obs.

Uncertainties for FQY and ϵ₃₄₇ are the standard deviation across measurements on three samples. Uncertainties for fitting results are the standard deviation across three successive data acquisitions.